Extracts from E.globulus that have phytochemical and antibacterial properties against pathogenic microbes such S. aureus, P. aeruginosa, E. coli, and S. pyogenes.The objective of this work was to ascertain the lowest inhibitory concentration of ethanol and aqueous leaf extracts on E. coli, P. aeruginosa, S. aureus, and S. pyogenes, as well as to analyze the phytochemical and antibacterial activities of E.globulus extracts. After being gathered from the Hosanna area, E.globulus leaves were taken to the Wachemo University Biotechnology lab for additional processing.Plant extracts were weighed (100gm) for antibacterial activity and phytochemical analysis, and serial dilution was used to calculate the minimum inhibitory concentration (MIC). The SPSS program was used to do the analysis of variance. At a significance threshold of p<0.05, treatment means were compared.The presence of phytochemicals was demonstrated by a qualitative phytochemical screening test of ethanol and aqueous crude extract. The ethanol leaf extract demonstrated a greater zone of inhibition against P. aeruginosa (25.900±0.1mm), while the aqueous leaf extract demonstrated a significant zone of inhibition against S. pyogenes (21.4±0.1mm).In order to develop new antimicrobial medicines from E. globules plants, more study into the separation of bioactive compounds is therefore feasible.