Prostate carcinoma remains a leading cause of cancer-related morbidity and mortality among men in Uganda. Immunohistochemical markers such as Foxp3 and p53 have emerged as potential indicators of tumor aggressiveness, but their expression patterns in Ugandan prostate carcinoma blocks remain underexplored. To determine the immunohistochemical expression of Foxp3 and p53 and assess their correlation with histological grades in prostate carcinoma tissue blocks at the Uganda Cancer Institute. We conducted a cross-sectional study on formalin-fixed paraffin-embedded prostate carcinoma tissue blocks that were diagnosed between December 2024 to January 2021. Hematoxylin and eosin staining was used for histological grading, and immunohistochemistry was performed to detect Foxp3 and p53 expression. The correlation between markers’ expression and histological grade was analyzed using Spearman’s correlation test, and a p-value of ≤ 0.05 was deemed significant. Foxp3 expression was absent in 81.9% of blocks and detected in only 18.1%, predominantly in Grade Group 1 tumors. However, the association between Foxp3 expression and histological grade showed a negative correlation. In contrast, p53 was expressed in 100% of the tissue blocks analyzed and showed a statistically significant positive correlation with higher histological grade. The aggressiveness of prostate carcinoma was shown by overexpression of p53 and the absence of Foxp3 in most of the prostate tissue blocks. This study gives information on the relevance of Foxp3 and p53 expressions in prostate cancer diagnosis and prognosis in Uganda.